Delhi
phone icon in white color

Call Us

Book Free Appointment

USFDA Approved Procedures

USFDA Approved Procedures

Minimally invasive. Minimal pain*.

Minimally invasive. Minimal pain*.

Insurance Paperwork Support

Insurance Paperwork Support

1 Day Procedure

1 Day Procedure

What is Blastocyst Culture?

Following the egg retrieval process, IVF (in-vitro fertilisation) or ICSI (intra-cytoplasmic sperm injection) are used in the IVF lab to fertilise the eggs. The resulting embryos are then placed in an incubator for further development.

Overview

Blastocyst Culture-Overview
Blastocyst Development Stages
  • Egg retrieval
  • Pronuclear stage
  • Two to four cell stage
  • Eight cell stage
  • Morula stage
  • Blastocyst stage
Prevention & Tips
  • Consume foods rich in antioxidants and healthy fats
  • Avoid excessive sugar, caffeine, and alcohol
  • Consume an adequate amount of water
  • Ensure proper folic acid and prenatal vitamins consumption
Stages of Blastocyst Transfer
  • Apposition
  • Adhesion
  • Invasion
Blastocyst Culture Treatment Image

Stages of Blastocyst Development

  • Day 0: The egg retrieval stage. IVF or ICSI is done after egg retrieval.
  • Day 1: The pronuclear stage, when the embryologist determines the number of successfully fertilised eggs
  • Day 2: two to four cell stage
  • Day 3: eight-cell stage
  • Day 4: the morula stage
  • Day 5-6: the blastocyst embryo stage

 

Two distinct cell types are seen in the blastocyst: the inner cell mass, which develops into the foetus, and the outer layer of cells, known as trophectoderm, which develops into the placenta. 

 

Transferring embryos after IVF or ICSI has traditionally been done on the second or third day after egg collection. However, in modern times, we can now culture embryos for five or six days owing to the advancements in the IVF lab. Before day five or six, some embryos stop developing (arrest). The embryologist, therefore, uses natural selection to select the best embryo for embryo transfer.

Our Clinics in Delhi

Loading map...
Pristyn Care La Midas
Map-marker Icon

NR - 38, Main Nathupur Road, DLF Phase- 3, GURUGRAM, Haryana 122002

Doctor Icon
  • Hospital
Pristyn Care Elantis - Super Speciality Hospital | NABH Accredited
Map-marker Icon

29, Ring Road, Lajpat Nagar 4, New Delhi, Delhi 110024

Doctor Icon
  • Hospital

Our Hospitals

  • hospital image
    hospital image

    Pristyn Care Elantis

    4.7/5
    Reviews (105)
    location Address : 29, Ring Road, Block L, Lajpat Nagar, Delhi - 110024
    NABH NABH
    24x7 Open 24x7 Open
    emergency icon Emergency Care

    Pristyn Care Elantis is dedicated to revolutionising surgical care. We combine the latest medical technology with highly skilled professionals and a patient-centric approach to transform the healthcare experience. Our team of doctors at Pristyn Care Elantis, strives to simplify the entire patient journey, ensuring a seamless process from diagnosis through recovery.

    We offer specialised treatments across a wide range of medical fields, including proctology, laparoscopic surgery, ENT (ear, nose, and throat), vascular, gynaecology, urology, aesthetics, orthopaedics, ophthalmology, and weight loss solutions.

    The Suites & Rooms at Pristyn Care Elantis are designed with your comfort in mind, offering a welcoming ambiance and top-notch amenities to make your stay as pleasant as possible.

    Our highly skilled medical team, supported by advanced infrastructure, makes us a trusted choice for comprehensive healthcare solutions.

    ... 

    Read More

    top specialities
    Proctology
    Urology
    Laparoscopy
    8 + More
  • hospital image
    hospital image

    Pristyn Care Diyos

    4.7/5
    Reviews (74)
    location Address : A1/26, adjacent to Green Fields Public School, Safdarjung Enclave, Delhi - 110029
    NABH Certified Listing NABH
    24x7 Open 24x7 Open
    emergency icon Emergency Care

    Pristyn Care Diyos Super-Speciality Hospital is committed to redefining modern healthcare by blending cutting-edge technology with compassionate, patient-first care. Located in the heart of Safdarjung Enclave, South Delhi, our NABH-accredited facility offers advanced treatment solutions across multiple specialties, ensuring superior outcomes and faster recoveries.

    Our dedicated team of doctors at Pristyn Care Diyos Hospital aims to streamline the entire patient journey—from consultation to recovery—by providing seamless, end-to-end medical care tailored to each individual’s needs.

    We offer specialised medical services across a wide range of fields, including urology and andrology, general and laparoscopic surgery, cosmetic and plastic surgery, gynaecology and urogynaecology, orthopaedics, neuro and spine surgery, ENT (ear, nose, and throat), oncology, internal medicine, and critical care. Our facility also extends home care services for enhanced convenience and continuity of care.

    The suites and patient rooms at the Hospital are thoughtfully designed to ensure a comfortable and healing environment, featuring modern amenities, hygienic surroundings, and attentive support for both patients and their attendants.

    Supported by a skilled clinical team and world-class infrastructure—including modular operation theatres with HEPA filters, advanced diagnostic labs, and high-end medical equipment— Pristyn Care Diyos Hospital stands as a trusted destination for safe, effective, and personalised healthcare.

    ... 

    Read More

    top specialities
    ENT
    Aesthetics
    Gynaecology
    7 + More
  • hospital image
    hospital image

    Pristyn Care La Midas

    4.7/5
    Reviews (66)
    location Address : Main, Nathupur Rd, nr. 38, DLF Phase 3, Sector 24, Gurgaon - 122002
    24x7 Open 24x7 Open

    Pristyn Care La Midas  is an award-winning, internationally recognised Super Specialty Hospital located in Gurugram, Haryana. We are dedicated to delivering the highest quality aesthetic services, combining advanced medical technology with a compassionate, patient-first approach.

    Our state-of-the-art facility offers a comprehensive range of surgical and non-surgical treatments for skin, hair, and body, all at affordable prices. We specialise in services such as laser hair removal, skin rejuvenation, body contouring, and hair restoration, ensuring that each patient receives personalised care tailored to their unique needs.

    At Pristyn Care La Midas Hospital, we prioritise patient comfort and satisfaction throughout the treatment journey. Our experienced team of medical professionals is committed to helping you achieve your beauty and wellness goals in the most natural and effective ways possible.

    Experience the perfect blend of luxury and innovation at Pristyn Care La Midas Hospital, where your well-being is our top priority.

    ... 

    Read More

    top specialities
    Laparoscopy
    Urology
    Proctology
    8 + More
  • hospital image
    hospital image

    Pristyn Care Ferticity

    4.7/5
    Reviews (34)
    location Address : 12, Navjeevan Vihar, Geetanjali Enclave, Malviya Nagar, Delhi - 110017
    Mon-Sat: 9:30 am-6:30 pm Mon-Sat: 9:30 am-6:30 pm

    Located in South Delhi, our facility is spacious, serene, and designed to make couples feel comfortable throughout their treatment. We pride ourselves on a warm, clean environment, offering easy access and convenient parking.

    With a reputation built on the strength of our experienced clinical team, we specialise in customised treatment protocols and provide comprehensive care, from the initial consultation to treatment completion. Pristyn Care Ferticity Super Specialty & IVF Hospital has helped over 40,000 families worldwide realise their dream of parenthood, maintaining the highest success rates in the field.

    Our clinic ensures a partnership between patients, doctors, and staff, delivering compassionate care every step of the way.

    ... 

    Read More

    top specialities
    Fertility
    Gynaecology

Why Pristyn Care?

Delivering Seamless Surgical Experience in India

01.

Pristyn Care is COVID-19 safe

Your safety is taken care of by thermal screening, social distancing, sanitized clinics and hospital rooms, sterilized surgical equipment and mandatory PPE kits during surgery.

02.

Assisted Surgery Experience

A dedicated Care Coordinator assists you throughout the surgery journey from insurance paperwork, to free commute from home to hospital & back and admission-discharge process at the hospital.

03.

Medical Expertise With Technology

Our surgeons spend a lot of time with you to diagnose your condition. You are assisted in all pre-surgery medical diagnostics. We offer advanced laser and laparoscopic surgical treatment. Our procedures are USFDA approved.

04.

Post Surgery Care

We offer free follow-up consultations and instructions including dietary tips as well as exercises to every patient to ensure they have a smooth recovery to their daily routines.

Frequently Asked Questions

How is blastocyte culture cost determined in Delhi?

The blastocyte culture cost varies significantly from region to region. The cost is usually an additional charge on the standard IVF procedures, and embryo transfer cost. Also, factors including the hospital’s reputation and precise location in Delhi, the specific technology used, overall IVF package cost are the deciding factors, too.

What is blastocyst culture in IVF?

The embryologist can choose the healthiest embryos through blastocyst culture IVF, which raises the likelihood of pregnancy. The embryos must be cultured in a laboratory until they reach the blastocyst stage before being placed in the uterus.

What are the blastocyst implantation stages?

Three blastocyst implantation stages, include, apposition, adhesion, and implantation . For better understanding, read them above.

What to expect in a 5 day blastocyst implantation timeline ivf?

In a day-5, IVF blastocyst transfer, the implantation procedure usually begins 1-2 days post-transfer as the embryo hatches and attaches to the uterine lining. The implantation can take around 5 days to complete.

Benefits of blastocyst culture and transfer 

  • The best possible laboratory conditions are necessary for blastocyst culture. Approximately 45% of fertilised eggs usually develop into blastocysts. 
  • Only the best developing embryos become blastocysts. The most suitable embryo or embryos for embryo transfer can be chosen by letting the embryos grow in the lab until they reach the blastocyst stage.
  • Additionally, in a natural pregnancy, the embryo takes around five days after fertilization to reach the womb. On this day, the uterine lining is very responsive. Therefore, blastocyst transfer is more of a physiological process, hence. 

 

Embryo biopsy is advised during the blastocyst development stage rather than on Day 2 or Day 3 embryos, making blastocyst culture necessary.

 

Who Can Benefit from Blastocyst Culture?

Blastocyst culture is advised for:

 

  • Multiple failed IVFs: Using a blastocyst culture, for individuals with a history of unsuccessful IVF with day 2/3 embryo transfers can help increase the likelihood of a successful pregnancy and provide further information about the embryos’ progress
  • Preventing multiple pregnancies: Blastocyst culture lowers the risk of multiple pregnancies by enabling the transfer of the single best embryo

 

Why is Blastocyst culture recommended?

The blastocyst culture is preferred in the following cases:-

 

  • High Success Rate: It has a higher success rate compared to the day 3 embryo transfer. Therefore, it is specifically advised to people who have had multiple IVFs and ICSI failures, despite having several good quality eggs. 
  • Elective Single Embryo Transfer(eSET): Day 5 Blastocyst transfer is extremely advantageous for those couples who are seeking the eSET option. As it increases the possibility of conceiving as compared to the commonly performed Day 3 embryo implantation for a single embryo. 
  • Preimplantation Genetic Diagnosis(PGD): PgD uses embryo biopsy, to carry out a genetic analysis. It is extremely beneficial for the couples, where one or both the partners have a genetic condition and they don’t want to pass it onto the child. Embryo biopsy allows only the healthy embryos to be transferred to the uterus, after careful selection. 
  • Multiple healthy embryos: In the cases of multiple healthy embryos, embryologist can select the best embryo, through carefully observing the frozen embryo developing into the blastocyst. 

 

Stages

 

Apposition

It is the initial unstable position of the blastocyte close to the endometrial epithelium, usually in the fundal or posterior portion of the uterus. The inner cell mass (ICM) of the blastocyst aligns itself with the uterine surface at this phase. Through weak electrostatic and biochemical interactions mediated by microvilli, the embryo is able to explore the endometrial surface before forming a strong connection.

 

Adhesion 

The blastocyst adheres to the luminal epithelium in a steady manner after apposition. Cell adhesion molecules like integrins, selectins, and cadherins establish a strong connection between trophoblastic cells and endometrial epithelial cells, facilitating this phase. In turn, the endometrium needs to be in a receptive state, which is sometimes referred to as the window of implantation and is controlled by the oestrogen and progesterone hormones.

 

Invasion

The trophoblast develops into two separate layers after adhering firmly:

  • Cytotrophoblast (inner layer of proliferation)
  • Syncytiotrophoblast (the outer layer of invasion)

 

The syncytiotrophoblast penetrates the stromal tissue beneath the uterine epithelium. In order to enable early uteroplacental circulation, this phase entails significant remodelling of the maternal extracellular matrix and vascular. Immune modulation systems and proteolytic enzymes enable this controlled invasion, ensuring the semi-allogeneic embryo’s tolerance.

Read More
Disclaimer: **The result and experience may vary from patient to patient.. ***By submitting the form or calling, you agree to receive important updates and marketing communications.

© Copyright Pristyncare 2025. All Right Reserved.